Microbeads
Memory B Cell Isolation Kit, human
The Memory B Cell Isolation Kit has been developed for the isolation of memory B cells from human PBMCs.
Quantity
Background information
Memory B cells, defined as quiescent antigen-experienced B cells, are generated in response to T cell–dependent and T cell–independent antigens. They are able to react quickly to a recurrent antigenic challenge, thereby providing serological immune protection.
CD27, a member of the TNF receptor family, is expressed on most memory B cells. It allows their discrimination from naive B cells, which are CD27
–
.
Detailed separation procedure
Memory B cells are isolated by depletion of unwanted cells and subsequent positive selection with CD27 MicroBeads. Unwanted cells, for example, T cells, NK cells, monocytes, dendritic cells, granulocytes, platelets, and erythroid cells are depleted using a cocktail of biotinylated antibodies against CD2, CD14, CD16, CD36, CD43, and CD235a (glycophorin A), and Anti-Biotin MicroBeads.
Columns
For the first magnetic separation (depletion): LD or autoMACS
®
Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.