First, the Ly-6B.2 (7/4)
+
cells are magnetically labeled with Anti- Ly-6B.2 (7/4) MicroBeads. Then, the cell suspension is loaded onto a MACS
®
Column, which is placed in the magnetic field of a MACS Separator. The magnetically labeled Ly-6B.2 (7/4)
+
cells are retained within the column. The unlabeled cells run through; this cell fraction is thus depleted of Ly-6B.2 (7/4)
+
cells. After removing the column from the magnetic field, the retained Ly-6B.2 (7/4)
+
cells can be eluted as the positively selected cell fraction. To increase the purity of spleen cells, the positively selected cell fraction containing the Ly-6B.2 (7/4)
+
cells must be separated over a second column.