Isolation or depletion of CD4
+
T helper cells is performed in many different fields of research, such as infectious diseases, autoimmune diseases, allergy, asthma, and tumor immunology. T helper cells isolated by MACS® Technology remain viable and functional. Therefore, they can be used for further functional studies, such as proliferation assays
and cytotoxicity assays
, as well as for the generation of T cell lines
. CD4
+
T cells purified with CD4 MicroBeads have also been cultured and analyzed for
in vitro
cytokine production.
Furthermore, they have been used in co‑culture experiments, e.g., for allogeneic stimulation by dendritic cells
or for B cell activation
.