Microbeads
Basophil Isolation Kit II, human
The Basophil Isolation Kit II is an indirect magnetic labeling system for the negative isolation of basophils from PBMCs.
Quantity
Detailed separation procedure
Non-basophils (i.e. T cells, NK cells, B cells, monocytes, dendritic cells, erythroid cells, platelets, neutrophils, and eosinophils) are indirectly magnetically labeled using a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. Isolation of highly pure basophils is achieved by depletion of the magnetically labeled cells.
Downstream applications
Isolated basophils have been used for phenotypical characterization
3
, for histamine, leukotriene, and cytokine
1, 4, 5, 6
release assays, for molecular studies
1, 2, 5
, protein analysis
4, 5
, and other applications.
Columns
LS, XS, or autoMACS
®
Columns.