Detailed separation procedure
Non-basophils (i.e. T cells, NK cells, B cells, monocytes, dendritic cells, erythroid cells, platelets, neutrophils, and eosinophils) are indirectly magnetically labeled using a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. Isolation of highly pure basophils is achieved by depletion of the magnetically labeled cells.