All media used in tissue culture have a basis of a synthetic
mixture of inorganic salts known as a physiological
or balanced salt solution (BSS). All the physiological
salt solutions have been derived from the salt solution
originally described by Sydney Ringer (1885). The first
balanced salt solution to be developed specifically for
supporting the metabolism of mammalian cells was
Tyrode's solution. Since then many modifications have
been done to obtain better buffering salt solutions and to
prevent calcium precipitation.
The function of a salt solution is:
·To maintain the medium within physiological pH range.
·To maintain intracellular and extra cellular osmotic
balance.
·Modified with a carbohydrate, such as glucose serves as
an energy source for cell metabolism.
TL1006 is 1X Dulbecco's Phosphate Buffered Saline
without calcium, magnesium and phenol red. It is most
commonly used for tissue disaggregation and monolayer
dispersal since presence of calcium and magnesium ions
may hinder the trypsin activity.