Ham's Nutrient Mixtures were originally developed for
single cell plating of near diploid Chinese hamster ovary
(CHO) cells and mouse L-cells. Both F-10 and F-12 are
formulated for use with or without serum, depending on
the type of cells being cultured.
Ham's Nutrient Mixture F-10 was designed for clonal
growth of CHO cells and chick embryo cells under serum
free conditions. It is now widely used for culturing a
variety of cells which include human diploid cells and
white blood cells for chromosomal analysis and primary
explants of rat, rabbit and chicken tissues.
AL024 is Nutrient Mixture F-10 Ham with sodium
bicarbonate. It does not contain L-glutamine. Users are
advised to review the literature for recommendations
regarding medium supplementation and physiological
growth requirements specific for different cell lines