Molecular Weight: 371.39
Molecular Formula: C16H18N3NaO4S
CAS No: 69-52-3
Synonym: D(-) α-Aminobenzylpenicillin.Na
Ampicillin belongs to the penicillin group of b-lactam
antibiotics. It differs from penicillin by the presence
of an amino group. It inhibits bacterial cell-wall
synthesis (peptidoglycan cross-linking) by inactivating
transpeptidases on the inner surface of the bacterial cell
membrane. This broad spectrum antibiotic is effective
against Gram-positive and Gram-negative bacteria.
In cell culture, ampicillin is used as an antibacterial agent
and as a selection agent to select transformed bacteria.
Many common vectors carry genes encoding resistance
to ampicillin and are identified by the ability of the
host bacteria to grow in the presence of this antibiotic.
Resistance is mediated by cleavage of β-lactam ring of
ampicillin by β-lactamase.
In cell culture applications ampicillin sodium salt is
generally used at a concentration of 100mg/L for
antibacterial use. In ampicillin resistance studies, it is
used at a concentration of 20-125µg/ml.
A022 is sterile filtered solution formulated to contain
10mg ampicillin per ml