Microbeads
B Cell Isolation Kit II, human
The B Cell Isolation Kit II was developed for the fast and efficient isolation of untouched B cells from human PBMCs.
Quantity
Detailed separation procedure
Non-B cells (T cells, NK cells, monocytes, dendritic cells, granulocytes, platelets, and erythroid cells) are labeled with a cocktail of biotinylated CD2, CD14, CD16, CD36, CD43, and CD235a (glycophorin A) antibodies. These cells are subsequently magnetically labeled with Anti-Biotin MicroBeads for depletion. Highly pure B cells are obtained by depletion of magnetically labeled non-B cells.
Downstream applications
Untouched B cells obtained with the B Cell Isolation Kit II can be used for the isolation of specific B cell subsets, including naive and memory B cells.
1,2,3Columns
MS, LS, XS, or autoMACS
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