Intermediate and non-classical monocytes together are referred to as CD16

 monocytes

 and account for about 5–10% of all blood monocytes. CD14

CD16

 monocytes differ from CD14

CD16

 monocytes with regard to phenotype and immunological function. CD16

 monocytes are, for example, considered more mature and are believed to have a higher T cell stimulatory capacity than CD16

 monocytes.

Isolation of CD16

 monocytes is performed in a two-step procedure. Prior to separation, cells are incubated with FcR Blocking Reagent. Granulocytes and NK cells are then magnetically labeled with a cocktail of CD15 MicroBeads and CD56 MicroBeads, and depleted over a MACS Column. The flow-through in this first separation step contains pre-enriched, unlabeled CD16

 monocytes. In the second separation step, CD16

 monocytes are efficiently isolated by positive selection after labeling with CD16 MicroBeads.

Isolated blood CD16

 monocytes are suitable, for example, for investigations on CD16

 monocyte maturation, migration, and differentiation, or for studies on antigen uptake, antigen processing and presentation to T cells.

For the first magnetic separation (depletion): LD or autoMACS

 Columns. For the second magnetic separation (positive selection): MS, LS, or autoMACS Columns.