Microbeads
Anti-MHC class II (Ia) MicroBeads, mouse
Mouse Anti-MHC Class II MicroBeads are used for the positive selection or depletion of MHC class II+ cells from lymphoid and non-lymphoid tissue or from blood.
Quantity
Overview
Mouse Anti-MHC Class II MicroBeads are used for the positive selection or depletion of MHC class II
+
cells from lymphoid and non-lymphoid tissue or from blood.
Detailed product information
Background information
MHC class II is expressed on B cells, macrophages, monocytes, dendritic cells, thymic epithelial cells, and on subsets of hematopoietic progenitor cells. The MicroBeads bind to MHC class II haplotypes I-A
b
, I-A
d
, I-A
q
, I-E
d
, and I-E
k
, which are expressed by the most common inbred mouse strains, e.g. C57BL/6, BALB/c, or 129/SvEv. MHC II haplotypes I-A
f
, I-A
k
, and I-A
s
are not recognized.
Downstream applications
Isolated MHC class II
+
cells are suitable for studies on antigen presentation such as in T cell stimulation assays. Anti-MHC Class II MicroBeads were used for positive selection of Langerhans cells from epidermis
, for the isolation of untouched T cells from lymph nodes by depletion of MHC class II
+
cells
, or in combination with other MicroBeads for isolation of untouched T cells from spleen
.
Columns
For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.