Columns
MACS ART MS Column (six columns)
The MACS ART Annexin V System is intended for the in vitro depletion of apoptotic human spermatozoa. The system selectively depletes unwanted cells using the natural ability of Annexin V to recognize the antigen EPS (externalized phosphatidylserine) in the plasma membrane of apoptotic cells.
Phosphatidylserine-positive cells are efficiently depleted from fresh, cryopreserved, or otherwise manipulated semen samples. The yielded spermatozoa fraction may be used in subsequent operations in Assisted Reproductive Technology.
The enriched sperm populations show higher overall quality in terms of: morphology, motility, DNA fragmentation, apoptosis, cryosurvival rates, and fertilization potential.
Quantity
Annexin V recognizes an antigen (externalized phosphatidylserine, EPS) in the plasma membrane of apoptotic cells.
Apoptotic cell depletion begins with the magnetic labeling of apoptotic cells by the MACS
®
ART Annexin V Reagent. The labeled cells are then passed through a separation column located in a fixed magnetic field. Unwanted cells are selectively retained in the column. Living spermatozoa are not labeled by the reagent, so they pass through the column and are collected for later use.
The MACS ART Annexin V System components are free of human and animal-derived components. Product specifications are confirmed by batch-specific certificates of analysis and ensure consistency of quality. The MACS ART Annexin V System shows no embryo toxicity, according to the Mouse Embryo Assay (MEA).