HiGlutaXL™ medium contains the stabilised dipeptide form of L-glutamine, L-alanyl-L-glutamine. HiGlutaXL™ medium offers several advantages over the conventional glutamine containing media. Dipeptide form prevents the intramolecular cyclization reaction, thus preventing toxic build-up of ammonia. L-alanyl-Lglutamine incorporates L-alanine that protects the alpha amino acid group. Aminopeptidases within the cell break the dipeptide, gradually releasing both L-glutamine and L-alanine for use by the cell. The gradual release of Lglutamine obviates the need of addition of L-glutamine at the time of use and allows liquid media to be stored at 4°C for longer periods. AL047G is HiGlutaXL™ Minimum Essential Medium Eagle with Earle's salts, L-alanyl-L-glutamine, sodium bicarbonate and non-essential amino acids. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.