Microbeads
Anti-Ly-6B.2 (7/4) MicroBeads, mouse
The Anti-Ly6B.2 (7/4) MicroBeads, mouse can be used for isolation of neutrophils, inflammatory monocytes or myeloid progenitors from bone marrow or spleen.
Quantity
Background information
- Positive selection or depletion of cells expressing mouse Ly-6B.2 antigen from bone marrow and spleen.
- Isolation of recruited inflammatory myeloid cells in experimental mouse models. The antigen is only expressed on mouse strains of the Ly6.2 haplotype.
Detailed separation procedure
First, the Ly-6B.2 (7/4)
+
cells are magnetically labeled with Anti- Ly-6B.2 (7/4) MicroBeads. Then, the cell suspension is loaded onto a MACS
®
Column, which is placed in the magnetic field of a MACS Separator. The magnetically labeled Ly-6B.2 (7/4)
+
cells are retained within the column. The unlabeled cells run through; this cell fraction is thus depleted of Ly-6B.2 (7/4)
+
cells. After removing the column from the magnetic field, the retained Ly-6B.2 (7/4)
+
cells can be eluted as the positively selected cell fraction. To increase the purity of spleen cells, the positively selected cell fraction containing the Ly-6B.2 (7/4)
+
cells must be separated over a second column.
Columns
MS, LS, or autoMACS
®
Columns.