Microbeads
CD45RO MicroBeads, human
CD45RO MicroBeads were developed for positive selection or depletion of lymphocyte subsets from human PBMCs.
Quantity
CD45RO is expressed on CD4
+
and CD8
+
T memory cells as well as on CD4
+
effector T cells. CD45RO is also expressed on monocytes, macrophages, and granulocytes. The CD45RO antibody recognizes a 180 kDa isoform of the leukocyte common antigen (LCA).
CD45RO MicroBeads can be used for positive selection or depletion of CD45RO
+
cells. For isolation of CD45RO
+
T cell subsets, the CD45RO MicroBeads can be combined with CD4 and CD8 MACS® T Cell Isolation Kits
or MultiSort Kits
. The CD45RO MicroBeads can also be used to isolate CD45RA
+
cells from pre-sorted CD4
+
or CD8
+
T cells by depletion of CD45RO
+
cells.
Downstream applications
Cells isolated with CD45RO MicroBeads can be used for various studies, such as cytokine expression
or receptor signaling.
Columns
For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.