Detailed separation procedure
The isolation of mouse plasma cells is performed in a two-step procedure. First, non-plasma cells are indirectly magnetically labeled with a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. The labeled cells are subsequently depleted by separation over a MACS® Column. In the second step, plasma cells are directly labeled with CD138 MicroBeads and isolated by positive selection from the pre-enriched cell fraction. The magnetically labeled plasma cells are retained on the column and eluted after removal of the column from the magnetic field. To achieve highest purities, the positively selected cell fraction, containing the plasma cells, is separated over a second column.