Recombinant antibody

Clone REA304 recognizes the mouse CD83 antigen, a Single-pass type I membrane protein which belongs to the immunoglobulin superfamily. Murine CD83 is moderately expressed on resting T cells and DC, but strongly increases in its expression on T cells following activation with antigenic peptides or T cell receptor-specific monoclonal antibody. When returning to the resting state, T cells down-regulate CD83 again. Histological analysis of serial spleen sections revealed a CD83 expression pattern resembling that of MIDC-8, a known murine DC marker molecule.

Additional information: Clone REA304 displays negligible binding to Fc receptors.

The REA Control (I) antibody clone REA293 is a universal isotype control that can be used with all recombinant engineered antibodies (REAfinity™ Antibodies) that recognize intracellular antigens. REAfinity Antibodies have been engineered for their high specificity and contain human IgG1 parts for constant regions. Unspecific interactions of the fluorochrome can be controlled for with conjugated versions of clone REA293. For intracellular stainings however the isotype control staining should be combined with other negative controls (e.g. staining of unstimulated cells).

The REA Control (I) antibody clone REA293 is a universal isotype control that can be used with all recombinant engineered antibodies (REAfinity™ Antibodies) that recognize intracellular antigens. REAfinity Antibodies have been engineered for their high specificity and contain human IgG1 parts for constant regions. Unspecific interactions of the fluorochrome can be controlled for with conjugated versions of clone REA293. For intracellular stainings however the isotype control staining should be combined with other negative controls (e.g. staining of unstimulated cells).